Laboratory of Plants Introduction

Updated: 10/1/2020

The plant introduction laboratory was established at the Agrobiostation-Botanical Garden of Kherson State University in 2003.

Contact phone number of the laboratory: (0552) 37-66-27, mobile.0681141561



Head of the laboratory:

Mikhail Boyko-professor of the Department of Botany of KHSU, doctor of biological sciences, professor

Laboratory researchers:

Nina Sushinskaya-director of the AGROBIOSTATION-Botanical Garden of KHSU,

Pavlova Nadezhda Romanovna, candidate of Biological Sciences, Associate Professor of the Department of Botany of KHSU,

Vasily Derevyanko, candidate of Biological Sciences, leading researcher of the state research farm "Novokakhovskoe" of the Nikitsky Botanical Garden of the National Research Center,

Lyudmila V. Svidenko, candidate of Biological Sciences, researcher of the state research farm "Novokakhovskoe" of the Nikitsky Botanical Garden of the National Research Center,

Frantsanova Elena Viktorovna, post-graduate student of the Department of Botany of KHSU


  •  protection and conservation of genetic resources of plant species ex situ-in vivo (in the form of live collections);
  •  introduction and testing of promising species, forms and varieties of ornamental plants in culture;
  •  reproduction of relics, endems, rare and endangered species of natural flora in artificial conditions;
  •  creating and maintaining a Bank of ornamental plant seeds;



Report on the work of the laboratory for 2018-2019 report 2019.doc (3.9 Mb)

The collective monograph "checklist of plants and fungi of the Botanical Garden of Kherson State University" was published. . Boyko M. F.-Kherson: Aylant, 2011-104 P. the publication is dedicated to the 75th anniversary of the foundation of the Botanical Garden. Authors of the monograph: Boyko M. F., Derevyanko V. M., Derevyanko N. V., Zagorodnyuk N. V., Koroleva O. V., Melnik R. P., Moiseenko I. I., Sushinskaya N. I., Khodosovtsev O. E. This is the only catalog of the Botanical Garden, which contains information not only about gymnosperms and angiosperms (as is usually the case in the catalogues of other botanical gardens), but also about mosses, fungi and lichens.

Studies on the introduction of subtropical plants: Asimina, feijoa, pomegranate, fig, Persimmon, zizifus jujuba, avocado, tangerine, orange, Noble Laurel, Thunberg barberry and others. Today, we can note a high degree of adaptation of experimental plants to growing conditions. Shoots of figs and pomegranates, Albizia leonkoranskaya froze to soil level due to weather conditions in the winter of 2010-2011 and 2011-2012.figs and zizifus yuyuba bear abundant fruit despite adverse weather conditions. Studies on the vegetative propagation of home pomegranate have shown that cuttings from the side shoots and tops take root well, provided that the location is single and the diameter of the cuttings is from 0.5-1 cm. Cuttings in bundles rot as a result of infection with fungal diseases. Asimina cuttings take root well with medium doses of watering.

For the second year in a row, the fruits of the subtropical plant feijoa, which is a very valuable medicinal, ornamental and food crop, were obtained in closed ground conditions. Flowering was observed from the end of March to July, when moving the plant from the greenhouse to the open ground, partial fall of flowers and ovaries was observed. For better fertilization, artificial pollination of flowers with a brush and spraying of plants with water in the evening was carried out. Fruit fall was observed in the third decade of September and ended in the second decade of October. Fruits need to ripen. It is planned to collect and sow seeds for further research of the crop.

Experiments on the biological properties of coffee continue. Seedlings were purchased in the spring of 2009. In the first year, the growth of shoots was 15-25 cm, in the second year, the appearance of lateral shoots is observed. In 2011, in the Spring, Seeds of this type were purchased, which were sown in containers, shoots have not yet appeared, since the plant has a very long germination period - 16 months.

Study of the introduction of Thunberg Barberry – phenological observations, experiments on reproduction, the ability to adapt to growing conditions and determine seed germination. The anatomical and morphological structure of shoots of different ages and leaves is studied. Previous years ' data on the fruiting of the species have been confirmed. Abundant fruiting of almost all decorative forms except 'Bonanza gold' and 'Cobalt'was noted. It was found that seedlings of Berberis thunbergii DC begin to bear fruit in the fifth, and some even in the fourth year after sowing seeds. For the fourth year in a row, the appearance of self-seeding near the parent plants is observed, which indicates a high degree of Plant Introduction. In 2011, a large number of self-seeding was also noted. Seeds sown in 2010 in the greenhouse had a germination rate of up to 9 95. Up to 20℅ seedlings showed differences in morphological features relative to the parent plants. Experiments on vegetative reproduction of Berberis thunbergii DC continue in open (under arcs) and closed ground (in boxes in a greenhouse). At the same time, harvested cuttings that grew in the greenhouse before April form roots much faster, have greater growth of vegetative mass and more developed roots than those that did not grow in the greenhouse. Based on the preliminary results of the conducted experiments, it can be concluded that Berberis thunbergii DC and its decorative forms are really highly decorative, quite frost-resistant and drought-resistant species that can be successfully grown in the research area.

Introduced in 2008 varieties and forms of Cornus mas: Elena, Vladimirsky, elegant, gentle, Joy, Coral brand, Lukyanovsky, Kostya (National Botanical Garden named after N. N. Grishko of the National Academy of Sciences of Ukraine; November), No. 1 (Transcarpathian region; may) feel good, give growth, but need additional moisture. Phenological phases and drought resistance of Form No. 1 in the southern region were observed. In dry air conditions, the leaves dry out.

Research of ecological and biological features of newly introduced ornamental and medicinal essential oil crops. Medicinal essential oil crops were sown in two versions: directly in the open ground and to obtain seedlings in the greenhouse (in February). A very low percentage of germination was given by lavender seeds – 3 %, but all the resulting seedlings successfully transferred planting to the ground. Seeds of odorous Rue (Ruta graveolens L.), Hyssop officinalis (Hyssopus officinalis), Goatgrass officinalis (Galega Oficinalis L. it has a very high percentage of germination, and 100% of seedlings can withstand transplanting into the open ground. Experiments have been started to obtain essential oil from the following plants: clary sage, medicinal sage, Virgin Juniper, Santolina, medicinal hyssop and others.

The objects of research of the laboratory staff are also: ornamental climbing plants: Honeysuckle (4 types), passionflower (5 types); 3 types of sedge; 10 types of gymnosperms; bush plants: roses, honeysuckle, St. John's wort, pomegranate, fig, budlea; medicinal essential oil crops (10 types), pyracantha and others.

Experiments were conducted to compare the success of cuttings in open ground, closed ground (greenhouse) and under arcs with plastic wrap. In the greenhouse, some cuttings are placed in plastic containers with a hermetically sealed lid, some – directly in containers (plastic cups ). In open containers, cuttings were placed singly and in bundles.

Based on the results of scientific experiments conducted by the staff of the cuttings laboratory, conclusions were drawn, namely:: closed ground conditions, cuttings can be carried out throughout the entire winter period, which allows you to get planting material in a larger volume in a shorter period of time.

2.the highest percentage of rooting of cuttings is observed in closed ground conditions than in open ground conditions, namely in polyethylene containers with a hermetically sealed lid. is advisable to place cuttings singly, since cuttings in bundles that were in the middle did not form roots, and due to insufficient aeration were affected by fungal diseases.

Research has begun on a new crop – honey stevia, which was purchased in November 2010. Reproduction was tested by dividing the Bush, resulting in three plants. Observations of plants are made when propagating them by cuttings. In the open ground in summer, 50% of the cuttings took root. Adult plants had 6-7 shoots each, reaching 1.2 m in height. During the winter period, the plants are brought in containers in the greenhouse. An experiment on cuttings in winter in greenhouse conditions is laid. Seeds were obtained for laying a site of honey stevia in the spring and for continuing experiments on its introduction.

Seedlings of black walnut were obtained, the height of which by the end of the growing season is 10-15 cm, the seed germination rate is 40 %. 6 students – members of the circle "Biology" of the small Academy of Sciences-take part in scientific research.

In the spring of 2011, cuttings of Evergreen boxwood, cedar pine, Greek wrap, red honeysuckle, and tree hibiscus were carried out in the nursery on Maly Krug. During the season, 50% of boxwood, 100% of cedar pine, about 25% of wrap and only 5% of honeysuckle took root. The reason for such low rooting of the last two plants was insufficient moisture and a low level of agricultural technology.

Seeds of the following tree species were sown: tree Hazel, Red Oak, Stone Oak, European spruce, Western columnar Thuja, as well as four types of Oaks brought from Israel. In autumn, zizifus yuyuba, chekalkin walnut, black walnut were sown, seedlings of tree Hazel, horizontal Dogwood, 3-grade pyracantha, Holly Magonia, Julian barberry, Thunberg barberry were planted. Cuttings of hydrangeas, pyracantha, Santalina, caryopteris and repeated red honeysuckle and Greek Wrap were also carried out. Cuttings will grow in greenhouses. For the first time, a padded jacket plant was sown in a nursery on a small circle.

The collection of plants increased due to the seeds of the exchange fund: root processes of Ziziphus, frost-resistant pomegranate, early-maturing Dogwood variety, pecan seeds were obtained . Seeds of tetraploid Hawthorn, dogwood and other ornamental and food crops are collected.

According to the agreement on scientific cooperation with the Nikitsky Botanical Garden –The National Research Center, research continues on conducting experiments to determine the best rootstocks for Stone crops together with an employee of the Nikitsky Botanical Garden, Professor E. P. Chaferistov and his graduate students. In summer, cuttings of high – yielding varieties were grafted onto rootstocks-seedlings that are grown on the territory of the Agrobiostation-Botanical Garden.

From the Nikitsky Botanical Garden, the following plants were obtained and planted for research on the small Botanical circle, in greenhouses and on other sections of the AGROBIOSTATION-Botanical Garden of KHSU: seedlings of Virgin Persimmon, seeds of verginskaya Persimmon, Caucasian Persimmon, Japanese quince, Mountain Ash.

In addition to the Nikitsky Botanical Garden, the plant introduction Laboratory at the AGROBIOSTATION-Botanical Garden of KHSU has contacts with such well-known scientific institutions:

M. G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine (inst%/ua/net),
Botanical Garden of Odessa National University (,
Kryvyi Rih Botanical Garden of the National Academy of Sciences of Ukraine (),
Askania-Nova Arboretum,
Botanical Garden named after academician A.V. Fomin (,
Sofiyevka Arboretum”,
N. N. Grishko National Botanical Garden of the National Academy of Sciences of Ukraine
Environmental and educational activities of the laboratory.

Laboratory staff and students-practitioners in the specialty "Ecology "developed new excursion routes for schoolchildren of general education schools on the topics of excursions:" diversity of plants of the globe", "landscape model of the Kherson fortress in miniature"," decorative and floral plants", "diversity and use in gardening of indoor plants", "medicinal plants"," Lichenoindication role of lichens", etc. on the routes excursions are conducted for schoolchildren, students, disabled children and students of extracurricular circles.